kurstaki 0.5% wettable powder) at 6 g/l water, Neemraj Super (Az… The insecticides used are acetamiprid 20 SP (125 g/ha) and imidachloprid 17.8 SL (150 ml/ha). Its efficacy as a tool of biological control of mustard aphid, Lipaphis erysimi was tested under field conditions for two consecutive years. Mustard (Brassica) is the principal and popular oilseed crop in Bangladesh.This crop is infested by several species of insect pests of which the mustard aphid, Lipaphis erysimi (Kalt.) We have mostly made identifications from high resolution photos of living specimens, along with host plant identity. (Show World list). 4. J. Insect Sci. The aptera (see first picture below) has two rows of dark bands on the thorax and abdomen which unite into a single band near the tip of the abdomen. The swollen mustard aphids, Lipaphis erysimi, have been parasitised. @inproceedings{Mishra2003BioEcologyAM, title={Bio-Ecology And Management Of Mustard Aphid, Lipaphis Erysimi (Kali.) Of those aphid species, Baker (2015) lists 15 as occurring in Britain Chemical Control of Mustard Aphid, Lipaphis erysimi Kalt and Leaf Webber, Crocidolomia binotalis Zell on Cabbage. Lipaphis erysimi is confined to Britain and northern continental Europe. We especially thank Alan Outen, Bedfordshire Invertebrate Group, and Chris Shortall, Rothamsted Research, for permission to reproduce Alan's photo of a Lipaphis erysimi alate and for information about the colony. Adult mustard aphid, Lipaphis erysimi. A final transcriptome comprising 52,652 transcripts of 1064bp average length and N50 value of 1806 bp was generated. International … Plants remain stunted and sooty molds grow on the honey dew excreted by the insects. We fully acknowledge these authors as the source for the (summarized) taxonomic information we have presented. The wild crucifer aphid does not host alternate. A total of 20441 found to share no homology to any sequence available in the public domain and could therefore represent novel aphid genes or sequences that are unique to L. erysimi. This study reports the de novo transcriptome of L. erysimi for the first time. The mustard aphid, Lipaphis erysimi (Kalt. . We also present a comparative analysis of nymphs and adult transcriptomes to study the differential expression profiles associated with different developmental stages as well as different feeding conditions. Note the green bands on the back of the aphid. International Journal of Pest Management: Vol. The body length of Lipaphis erysimi alatae is 1.4-2.2 mm. Males have been found in some countries. The mustard or turnip aphid, Lipaphis erysimi pseudobrassicae (Davis), is one of the most destructive pests of brassica causing over 50% yield loss (Ronquist and Ahman 1990, Patel et al. A total of 27,112 transcripts were annotated with insect proteins from SwissProt, of which 4128 transcripts were components of 165 KEGG pathways. Lipaphis erysimi is a Brassicaceae specialist aphid, which causes significant losses in yield and/or reduction of oil content of vegetable and oilseed brassicas and is a major pest in the Indian subcontinent. Potential transcripts for host selection, detoxification, salivary proteins and effectors, molecular chaperones and developmental genes were identified. Comparative efficacy of insecticides against mustard aphid, Lipaphis erysimi (Kalt.). For this, RNA-seq was performed on three different biological samples adults, nymphs (with all nymph stages pooled) and adults starved for 3 hours (referred to as Adult Feeding, AF; Nymphs Feeding, NF, and Adult Starved_3 hr, ANF samples henceforth). with combination of … Lipaphis erysimi is usually regarded as a relatively common aphid in Britain, but until recently we have found it to be rather scarce in southern England. Singh PK, 2006. This is an exploratory study with no biological replicates. Intr oduction Mustard crop attacked by painted bug, mustard Saw fly, mustard aphid potato aphid, leaf miner, flea beetle, diamond-back moth, bihar hairy catterpiller, cabbage buter fly and tobacco caterpillar Among them, Mustard aphid. Modelling regional level spatial distribution of aphid (Lipaphis erysimi) growth in Indian mustard using satellite-based remote sensing data. Large aphid populations can affect leaf size and yield. However, the significant repertoire of feeding- and development-related genes and their differential expression profiles generated in this study adds to the limited data available on L. erysimi and it would facilitate studies on the molecular basis of aphid feeding and development. These however were mixed species colonies comprised of Lipaphis erysimi, Aphis fabae and Myzus persicae (see picture below). Enter multiple addresses on separate lines or separate them with commas. Keywords: Mustard aphid, Lipaphis erysimi, identification, life cycle, host crop and management 1. Lipaphis pseudobrassicae is a cosmopolitan and somewhat polyphagous aphid with preference to plant species in the mustard family. 15(2): 29-32. is one of the most damaging of biotic stresses that confront this crop. Determination of the amount of honeydew secreted by aphids in a specific crop variety is one of the best ways to assess the susceptibility of aphid infestation to the variety. 26: 343-345. The body length of adult Lipaphis erysimi apterae is 1.4-2.4 mm. This study reports the de novo transcriptome of L. erysimi for the first time. Then in mid-October 2018 we found colonies of wild crucifer aphid on seed heads of hedge mustard (Sisymbrium officinale, see picture below). Biocide Manic (Metarhizium anisopliae a.i. Mustard aphid, Lipaphis erysimi (Kaltenbach) is a major constraint in increasing the yield of rapeseed-mustard crops in India. These mixed-species colonies of aphids on the seedheads of shepherd's purse were still present up till late November. Micrographs of clarified mounts by permission of Roger Blackman, copyright AWP all rights reserved. Any errors in identification or information are ours alone, and we would be very grateful for any corrections. The mustard aphid, Lipaphis erysimi Kaltenbach, is a major pest in mustard growing regions of the wo rld. Lipaphis alliariae which has the antennal terminal process 1.2-1.35 the length of the siphunculi). NOTE: Your email address is requested solely to identify you as the sender of this article. Photo 2. Note the green bands on the back of the aphid. The more feeding of plant sap by aphid, the more secretion of honeydew. INTRODUCTION. Parasitized aphid (mummy) at bottom. Winged aphid in middle. Image 1327096 is of mustard aphid (Lipaphis erysimi ) adult(s) on cabbage. Several of the colonies were comprised mainly of immatures (see picture below). In the present study, RiD was highly upregulated in R. indica during aphid infestation initiating a defense system mediated by jasmonic acid (JA), but not by salicylic acid (SA)/abscisic acid (ABA). They are known as 'mummies'. The losses in yield by mustard aphid were 66-96% (Singh and Sachan, 1997). This is the subject of investigation. Blackman & Eastop list 15 species of aphid as feeding on those 3 species, and all Sinapis worldwide, and provide formal identification keys Entomologia experimentalis et applicata. Turnip aphid adults and immatures. (Hemiptera) and its inhibition by Rorippa indica defensin. It feeds on various different Brassicaceae, including Brassica, Raphanus, and Sinapis spp., although it is not usually found on field Brassica crops. Photo 3. It lives on the undersides of leaves as well as on inflorescences, young shoots and growing points. It is the most destructive of all the pests of mustard in Bangladesh (Alam et al., 1964; Ahmed et al., 1977; Haque & Miah, 1979) and reduces the yield of mustard considerably. Then in mid-October 2018 we found colonies of wild crucifer aphid on seed heads of hedge mustard (Sisymbrium officinale, see picture below). ), is a pest of cruciferous plants throughout the world but no information is available on its metabolic requirements or the composition of the honeydew. Short Communication. Response of mustard aphid, Lipaphis erysimi (Kalt.) Efficacy of newer insecticides was studied against mustard aphid, Lipaphis erysimi (Kalt.) Lipaphis erysimi is a Brassicaceae specialist aphid, which causes significant losses in yield and/or reduction of oil content of vegetable and oilseed brassicas and is a major pest in the Indian subcontinent. Lipaphis erysimi is usually regarded as a relatively common aphid in Britain, but until recently we have found it to be rather scarce in southern England. 2004; 66(2):30-32. crop in tropical and sub-tropical climate.Cultivated Brassica germplasm has failed so far to provide any source of resistance. The antennal terminal process is 0.8-1.15 times times the length of the siphunculi (cf. Lipaphis pseudobrassicae in which that combined length is usually more than 2.4 times as long as the siphunculus). The peak populations (42.7) and (28.7) per leaf on Rainbow and Oscar varieties were recorded. Mustard aphid, Lipaphis erysimi (L.) Kaltenbach is a perpetual annual threat in the cultivation of rapeseed- mustard (Brassica spp.) Lipaphis erysimi. Dilawari, V.K., and Atwal, A.S. 1989. It is by Alton N. Sparks, Jr. at University of Georgia. RH They are known as "mummies". Abstract. Adult apterae of Lipaphis erysimi are small to medium sized yellowish green, grey green or olive green aphids, with a faint white wax bloom. RiD was found to have an effective insecticidal property against mustard aphid, Lipaphis erysimi. Of those aphid species, Baker (2015) lists 21 as occurring in Britain 1977. Adult mustard aphid, Lipaphis erysimi. A total of 17,296 transcripts were classified based on their Gene Ontology. Of those aphid species, Baker (2015) lists 12 as occurring in Britain Mustard aphid (Lipaphis erysimi) and English grain aphid (Sitobion avenae) are among the most important pests in mustard and wheat fields in Nepal. MATERIALS AND METHODS The aphids were cultured on greenhouse grown radish plants. 66, No. On Mustard With Special Reference … Characterization of α-amylase and its activity from mustard aphid Lipaphis erysimi (Kalt.) 2004, Carter and Sorensen 2013, Adhab and Schoelz 2015). Mustard aphid: Lipaphis erysimi. Krishnaiah, K. and N. Jagan Mohan. The siphunculi are paler than the cauda and have dark tips. (Show World list). Except where otherwise specified, all text and images on this page are copyright InfluentialPoints under a Creative Commons Attribution 3.0 Unported License on condition that a link is provided to InfluentialPoints.com, Creative Commons Attribution 3.0 Unported License. The functional response of fourth instars, adult males and females of the sevenspotted lady beetle, Coccinella septempunctata Linnaeus (Coleoptera: Coccinellidae), was studied at different densities of aphid, Lipaphis erysimi (Kaltenbach) (Homoptera: Aphididae). The swollen mustard aphids, Lipaphis erysimi, have been parasitised. Thank you for your interest in spreading the word about bioRxiv. Adult winged mustard aphid, Lipaphis erysimi. was the most abundant species on the crop. 99-105. This aphid is globally distributed, located in all … Pacific Pests and Pathogens - Fact Sheets Common names. Department of Botany, University of Delhi, Department of Botany, Deshbandhu College, University of Delhi, De novo transcriptome profiling of mustard aphid (Lipaphis erysimi) and differential expression of transcripts associated with developmental stages, feeding and non-feeding conditions. 1979. Blackman & Eastop list 18 species of aphid as feeding on those 2 species, and all Raphanus worldwide, and provide formal identification keys Adult winged mustard aphid, Lipaphis erysimi. Common Name. Turnip aphid, Mustard aphid. Symptoms of damage: Both nymphs and adults suck the sap from leaves, buds and pods; Curling may occur in infested leaves and at advanced stage plants may wither and die; Plants remain stunted and sooty molds grow on the honey dew excreted by the insects; The infected field looks sickly and blighted in appearance The clarified slide mounts below are of adult viviparous female Lipaphis erysimi : wingless, and winged. (2020). (Show World list). The mustard aphid, Lipaphis erysimi (Kalt.) 2:103-108. Distribution. Despite the lateness of the year, there was no sign of any sexual forms and the aphids were reproducing viviparously (see picture below). Alatae (see second picture above) have a black head and thorax and a dusky green abdomen with black bands near the tip and conspicuous dark marginal sclerites. 2010-2016b; 18(2):333-335. Incidence of aphid during re productive stage of m ustard causes severe (Show British list). to allylisothiocyanate. revealed that aphid appeared on leaves during 3rd week of January and on the inflorescences during 2nd week of February and continued up to harvesting on both the varieties. Wild germplasm is a potential source of resistance against many threatening herbivores. Imidacloprid application, as a seed coating agent on oilseed rape, is recommended to control mustard aphid, Lipaphis erysimi (Kaltenbach) (Hemiptera: Aphididae). (Show British list). Keeping the lack of information on the efficacy of this fungus on this particular aphid species, it was proposed to study the management aspects of mustard aphid, Lipaphis erysimi by use of different dosages of this fungus. Indian J Ent. A total of 23,532 transcripts that remained unannotated were subjected to BLAST against aphid sequences available at AphidBase and a total of 3091 transcripts had hits with sequences of other aphids in the database, out of which 1380 had protein hits. Second image: Lipaphis erysimi alate, copyright Alan Outen & Rothamsted Research, all rights reserved. The infected filed looks sickly and blighted in appearance. (Show British list). Rohilla HR, Bhatnagar P, Yadav PR. has been found to be great hindrance and cause severe reduction to rapeseed and mustard. The combined length of antennal segment III and the terminal process is usually less than 2.4 times the length of the siphunculus (cf. = 1 × 109 spores/ml) at 3.3 ml/l water, Varunastra (Verticillium lecanii spores 2% aqueous suspension, 2 × 108 CFU/ml) at 6 ml/l water, Mahastra (Bacillus thuringiensis var. In the great majority of cases, identifications have been confirmed by microscopic examination of preserved specimens. This question is for testing whether or not you are a human visitor and to prevent automated spam submissions. Photo 2. Chemical control of mustard aphid with newer and conventional insecticides. is an important winter oilseeds crop of India.Mustard aphid, Lipaphis erysimi (Kalt.) The studies on the relative occurrence and abundance of mustard aphid, Lipaphis erysimi (Kalt.) Whilst we make every effort to ensure that identifications are correct, we cannot absolutely warranty their accuracy. Curling may occur in infested leaves and at advanced stage plants may wither and die. The cabbage aphid, Brevicoryne brassicae L. was also found in very small numbers. L. erysimi is a serious pest of mustard in India. Rapeseed and mustard are one of the most important oilseed crops in Jammu and Kashmir as they rank second to cereals. Resistance to mustard aphid infestation and its correlation to some physiological and biochemical traits in selected varieties of different Brassica species were studied. Then in late October we found much bigger colonies of Lipaphis erysimi on seedheads of shepherd's purse (Capsella bursa-pastoris). Blackman & Eastop list 25 species of aphid as feeding on Brassica species worldwide, and provide formal identification keys Annals of Plant Protection Sciences. Mustard aphid: Lipaphis erysimi: Symptoms of damage. and their effect on Coccinella septumpunctata was also recorded at Pilibhit (UP) during 2007-08 on Indian mustard. Both nymphs and adults suck the sap from leaves, buds and pods. Materials and Methods ‘Sarson”, B. rapa cv. Photo 3. Our particular thanks to Roger Blackman for images of his clarified slide mounts. Mustard aphid, Lipaphis erysimi (Kalt.) 2, pp. Pesticides. This could also allow identification of novel target genes for development of RNAi-based aphid control methods. We have used the keys and species accounts of Blackman & Eastop (1994) and Blackman & Eastop (2006) supplemented with Blackman (1974), Stroyan (1977), Stroyan (1984), Blackman & Eastop (1984), Heie (1980-1995), Dixon & Thieme (2007) and Blackman (2010). For assistance on the terms used for aphid morphology we suggest the figure provided by Blackman & Eastop (2006). BSH 1 and Indian mustard, Brassica juncea cv. Indian mustard (Brassica juncea L.Czern & Coss.) Introduction. (2008). Control of mustard aphid, Lipaphis erysimi (Kalt.) is very devastating. = 1 × 109 spores/ml) at 3 ml/l water, Agri Sakti (Beauveria bassiana a.i. The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. Aphids (Lipaphis erysimi Kaltenback) often reduce mustard (Brassica juncea L.) yield in India.This study was conducted to determine if intercropping with aromatic plant species could provide an environmentally safe method for aphid control. It is highly host specific, feeding exclusively on Brassica phloem sap. 6. Entomopathogenic fungi, Verticillium lecani has been considered to be pathogenic to a wide range of insect-pests including aphids, thrips and white fly (Brownbridge, 1995; Hall and Burges, 1979; Hall, 1982; Heyler, Gill, Baywater and Chambers, 992). Kawada, K. and T. Murai. Severe reduction to rapeseed and mustard are one of the siphunculi ) make! Of 27,112 transcripts were components of 165 KEGG pathways effectors, molecular and! Allow identification of novel target genes for development of RNAi-based aphid control Methods L. erysimi a. Methods ‘ Sarson ”, B. rapa cv, Carter and Sorensen 2013, Adhab and 2015... 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